If you need help using Excel, refer to our for instructions. Replace the plunger and push out most of the air, but do not crush your leaves. Safety: No matter how harmless the experiment, the safety is always a very important issue. The soap makes the leaf less hydrophobic and will help it more easily absorb the solution. This furtheraffected the results since the difference in the rates could have been due to the thicknessof the leaves or the different amount of the chlorophylls in a leaf. Procedure Remove the plunger from a syringe. Hypothesis Stomata are pores, typically found under the leaf lower epidermis , that control the gas exchange of transpiration, where water vapor leaves the plants, and carbon dioxide enters.
The rest of the energy is used to remove electrons from a substance such as water. Prepare the experiment by placing the water filled tank in front of the lamp then the beaker in front of the tank so that the light shines through the water tank, then to the beaker in order to keep the temperature controlled Figure 3 4. Hypothesis Stomata are pores, typically found under the leaf lower epidermis , that control the gas exchange of transpiration, where water vapor leaves the plants, and carbon dioxide enters. When all the disks have floated, try putting the cup in a dark cabinet or room, or cover the cup with aluminum foil. Check the cup after about fifteen minutes.
Objective : To investigate the relationship between different light intensity by having the source of light at different distance to the plant discs which is at; 10cm, 15cm, 20cm, 25cm, 30cm, and 35cm and rate of photosynthesis of plant. Light energy excites electrons that are carried down an electron transport chain. Plants use some of the oxygen they produce, but most of it is released. The disks are easier to count in the beaker, and there is more room for them to spread out rather than being piled on top of each other. All other environmental conditions were held constant for a period of 2 days.
The rate of photosynthesis is affected by a few of factors, including light intensity, temperature. The experimenter instructed the subject to write the alphabet backwards from Z to A within 30 seconds. Record the time it took for 5 leaf discs to float. In this research, the experiment will be investigated on whether the color of theleaves will affect the rate of photosynthesis. In plants, these proteins are held inside organelles called chloroplasts, while in bacteria they are embedded in the plasma membrane. Put 1-2 drops of ethanal in a test-tube and add about 2 cm3 of 2,4-dinitrophenylhydrazine solution. The two-tailed P value was less that0.
For each team of students, fill a beaker or cup about ¾ full with the detergent and sodium bicarbonate solutions. The experiment should continue until all the disks are floating. Because the leaf disks are living plant material that still require energy, they are simultaneously using O 2 gas during respiration and producing O 2 gas during photosynthesis. All of thedisks were cut with the same hole-puncher, ensuring they were all the exact same size. Results table: Potato chips cont. The answer is yes, some of the oxygen will be used in the leaf's respiration process. The reactions or properties to be investigated are as follows: A.
I predict that the stomatal density on high leafs is higher than on low leafs. Straws work best with spinach; hole punches work best with ivy. Next, 10 leaf disks from the dark green leaf were placed into eachbeaker. Arithmetic mean, Carbon dioxide, Leaf 1305 Words 6 Pages relationship of different wavelengths of light and the rate of photosynthesis in spinach leafs. Over the years, I have found the floating leaf disk assay technique to be reliable and understandable to students. Not only was the data compared by graphing the rate of photosynthesis, but alsothe unpaired data T-testing was programmed in order to further verify that there is asignificant statistical difference in the two rates of photosynthesis. There may be a couple of disks that you simply cannot dislodge.
The properties of lewis acid is to have high electron deficiency. You just watched photosynthesis occur! This could mean that substrate below. They should settle to the bottom of the cup see photos below. Therefore, sodium bicarbonate will be usedthroughout this lab in order to quicken the rate of photosynthesis. Release the vacuum so that the solution will enter the disks. The disks should sink to the bottom of the syringe when they are ready for the demonstration. Purpose: To measure the effect of light intensity on the rate of photosynthesis in the leaf cells.
It was hypothesized before the experiment that enough water with sunlight and air should make the plants grow healthy. This will create a larger surface area for carbon dioxide diffusion, the excretion of water vapor transpiration and the large amounts of oxygen being produced. This will cause oxygen to collect in the leaf tissue, causing it to rise. The class was divided into groups of 2. There were a few problems which occurred when this lab was being conducted. Introduction: In this experiment we learnt on carbonyl compounds, enols and enolates. The chloroplasts use light, an electromagnetic energy source, to produce food for the plant in the form of sugar molecules.
Part B Oxidation reactions A. Oxygen is produced as a by-product. The process was then repeated 4 times for the light green coloredleaf disks, then another 4 times for the red colored leaf disks. For eachof the 4 trials done per leaf, all of the leaves of each color were taken from the same plant. This extract is licensed under the.